The full-length genome sequence of a novel mitovirus from Botryosphaeria dothidea, the causal agent of pear ring rot disease

Here, we describe a novel mycovirus, tentatively designated as "Botryosphaeria dothidea mitovirus 3" (BdMV3), isolated from Botryosphaeria strain FJ, which causes pear ring rot disease in Fujian Province, China. The complete genome nucleotide sequence of BdMV3 is 2538 nt length and contains single 2070-nt open reading frame (ORF) encoding putative RNA-dependent RNA polymerase (RdRp) 689 amino acids (aa) using the fungal mitochondrial genetic code. BLASTp analysis revealed that RdRp shares 28.91%–69.36% identity (query coverage more than 90%) with those members genus Mitovirus, highest 69.36% 68.79% to corresponding aa sequences Rhizoctonia solani 10 Macrophomina phaseolina 4, respectively. Phylogenetic based on indicated new member Mitovirus family Mitoviridae.